Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antimetastatic Potentials of Dioscorea nipponica on Melanoma In Vitro and In Vivo

doi: 10.1155/2011/507920

Figure Lengend Snippet: The effects of DNE3 on cell viability, migration, motility, invasion, and adhesion of melanoma cells. (a) B16F10 and (b) HS68 cells were treated with DNE3 for 24 hours by MTT assay. (c) B16F10 cells were subjected to analyze for cell migration by wound healing assay. (d) Determined migration ability of B16F10 was subsequently quantified with that of control being 100% (without DNE3 for 48 h). Cells were treated with DNE3 for 24 hours and then subjected to analyze for (e and f) motility, invasion, and (g) adhesion as described in Materials and Methods. Results were statistically evaluated by using one-way ANOVA with post hoc Dunnett's test (*** P < .001). Results from 3 repeated and separated experiments were similar.

Article Snippet: Cell lines including A2058 from human melanoma, B16F10 from a mouse melanoma, and HS68 from a human foreskin fibroblast cells were obtained from American Type Culture Collection (Rockville, MD, USA) and cultured in DMEM (for A2058 and B16F10) or RPMI (for HS68).

Techniques: Migration, MTT Assay, Wound Healing Assay, Control

Journal: Oncotarget

Article Title: Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity

doi: 10.18632/oncotarget.6488

Figure Lengend Snippet: A. The yeast two-hybrid analysis was conducted using pPC86 (AD)/full-length human SGTA (derived from a normal heart cDNA library) and pDBLeu (BD)/full-length human REIC/DKK-3 plasmids. The blue colonies indicate those with an interaction between the two proteins. B. For the pull-down (PD) assay, the full-length cDNA of human REIC/DKK-3 and SGTA was cloned into the pFN21A and pMACS Kk.HA-C plasmids, respectively. Cell lysates from Halo-tagged REIC/DKK-3- and/or HA-tagged SGTA-transfected 293T cells were analyzed. The sample pulled down using Halo-tagged REIC/DKK-3 was analyzed by Western blotting (WB) using anti-HA antibody. C. REIC/DKK-3 and SGTA protein expression in 293T, PC3 and Hs68 cells was analyzed by Western blotting. Coomassie Brilliant Blue (CBB) staining of the membrane is shown as a loading control. D. The co-localization of REIC/DKK-3 and SGTA was examined by double immunofluorescence staining and observed by fluorescence microscopy. The images in green and red show the intracellular localization of REIC/DKK-3 and SGTA, respectively. The areas of overlap between REIC/DKK-3 and SGTA are shown in yellow in the merged image.

Article Snippet: The normal human foreskin fibroblast cell line Hs68 was provided by JCRB Cell Bank (Osaka, Japan).

Techniques: Derivative Assay, cDNA Library Assay, Clone Assay, Transfection, Western Blot, Expressing, Staining, Membrane, Control, Double Immunofluorescence Staining, Fluorescence, Microscopy

Journal: BMC Complementary and Alternative Medicine

Article Title: Protective effects and mechanisms of Terminalia catappa L. methenolic extract on hydrogen-peroxide-induced oxidative stress in human skin fibroblasts

doi: 10.1186/s12906-018-2308-4

Figure Lengend Snippet: The cytotoxicity and effect on intracellular oxidative stress in Hs68 of TCE. a Cell viability (%) of human fibroblasts (Hs68), human keratinocytes, and mouse melanoma cells treated with TCE; b Cell viability of Hs68 after treatment with TCE with or without 150 μM hydrogen peroxide (H 2 O 2 ) exposure. c Repressive effect of TCE on H 2 O 2 -induced intracellular oxidative stress in Hs68. Significant difference versus control: * p < 0.05; ** p < 0.01; *** p < 0.001. Significant inhibition versus H 2 O 2 -exposed group: # p < 0.05; ## p < 0.01

Article Snippet: Hs68, HaCaT cells, and B16F0 cells were purchased from the Bioresource Collection and Research Center in Hsinchu, Taiwan.

Techniques: Control, Inhibition

Journal: BMC Complementary and Alternative Medicine

Article Title: Protective effects and mechanisms of Terminalia catappa L. methenolic extract on hydrogen-peroxide-induced oxidative stress in human skin fibroblasts

doi: 10.1186/s12906-018-2308-4

Figure Lengend Snippet: Effect of TCE on the H 2 O 2 -induced ( a ) phosphorylation of mitogen-activated protein kinases, ( b ) c-Jun phosphorylation and c-Fos, ( c ) matrix metalloproteinase (MMP)-1, − 3, − 9, ( d ) hemeoxygenase-1 (HO-1), ( e ) cyclooxygenase-2 (COX-2) and ( f ) type I procollagen protein expressions in Hs68. Significant difference versus control: * p < 0.05; ** p < 0.01; *** p < 0.001. Significant inhibition versus H 2 O 2 -exposed group: # p < 0.05; ## p < 0.01; ### p < 0.001. EGCG: (−)-epigallocatechin gallate was used as a positive control

Article Snippet: Hs68, HaCaT cells, and B16F0 cells were purchased from the Bioresource Collection and Research Center in Hsinchu, Taiwan.

Techniques: Phospho-proteomics, Control, Inhibition, Positive Control

Journal: British Journal of Pharmacology

Article Title: WMJ‐8‐B, a novel hydroxamate derivative, induces MDA‐MB‐231 breast cancer cell death via the SHP‐1‐STAT3‐survivin cascade

doi: 10.1111/bph.13929

Figure Lengend Snippet: WMJ‐8‐B suppressed cell proliferation and affected cell cycle distribution in MDA‐MB‐231 cells. (A) Chemical structures of WMJ‐8‐A, WMJ‐8‐B, WMJ‐8‐C, WMJ‐8‐D, WMJ‐8‐E and WMJ‐8‐F. (B) 4T1, T47D, MCF‐7 and MDA‐MB‐231 cells were treated with vehicle or WMJ‐8‐A~F at 10 μM for 48 h. Cell viability was then determined by an MTT assay. Each column represents the mean ± SEM of six independent experiments performed in triplicate. Statistically significant differences were determined using the Kruskal–Wallis test. *P < 0.05, compared with the control group. Technical replicates were used to ensure the reliability of singe values for each experiment. (C) MDA‐MB‐231, MCF‐7, MCF‐10A and Hs68 cells were treated with vehicle or WMJ‐8‐B at the indicated concentrations for 24 h. Cell viability was then determined by an MTT assay. Each column represents the mean ± SEM of six independent experiments performed in triplicate. Statistically significant differences were determined using the Kruskal–Wallis test. *P < 0.05, compared with the control group. (D) Cells were treated with vehicle or WMJ‐8‐B at the indicated concentrations for 24 h. The acetylation status of H3 was then determined by immunoblotting. Each column represents the mean ± SEM of five independent experiments. Statistically significant differences were determined using the Kruskal–Wallis test. *P < 0.05, compared with the control group. (E) Cells were treated with vehicle or WMJ‐8‐B at the indicated concentrations for 24 h. The percentage of PI‐stained cells in subG1, G0/G1, S and G2/M phases was then analysed by flow cytometric analysis as described in the Methods section. Each column represents the mean ± SEM of five independent experiments. Statistically significant differences were determined using one‐way ANOVA, with Tukey's post hoc test. *P < 0.05, compared with the control group.

Article Snippet: Cell culture MDA‐MB‐231, MCF‐7, T47D, 4T1 and Hs68 cell lines were obtained from the Bioresource Collection and Research Centre (Hsinchu, Taiwan).

Techniques: MTT Assay, Control, Western Blot, Staining

Journal: British Journal of Pharmacology

Article Title: WMJ‐8‐B, a novel hydroxamate derivative, induces MDA‐MB‐231 breast cancer cell death via the SHP‐1‐STAT3‐survivin cascade

doi: 10.1111/bph.13929

Figure Lengend Snippet: WMJ‐8‐B suppressed cell proliferation and affected cell cycle distribution in MDA‐MB‐231 cells. (A) Chemical structures of WMJ‐8‐A, WMJ‐8‐B, WMJ‐8‐C, WMJ‐8‐D, WMJ‐8‐E and WMJ‐8‐F. (B) 4T1, T47D, MCF‐7 and MDA‐MB‐231 cells were treated with vehicle or WMJ‐8‐A~F at 10 μM for 48 h. Cell viability was then determined by an MTT assay. Each column represents the mean ± SEM of six independent experiments performed in triplicate. Statistically significant differences were determined using the Kruskal–Wallis test. *P < 0.05, compared with the control group. Technical replicates were used to ensure the reliability of singe values for each experiment. (C) MDA‐MB‐231, MCF‐7, MCF‐10A and Hs68 cells were treated with vehicle or WMJ‐8‐B at the indicated concentrations for 24 h. Cell viability was then determined by an MTT assay. Each column represents the mean ± SEM of six independent experiments performed in triplicate. Statistically significant differences were determined using the Kruskal–Wallis test. *P < 0.05, compared with the control group. (D) Cells were treated with vehicle or WMJ‐8‐B at the indicated concentrations for 24 h. The acetylation status of H3 was then determined by immunoblotting. Each column represents the mean ± SEM of five independent experiments. Statistically significant differences were determined using the Kruskal–Wallis test. *P < 0.05, compared with the control group. (E) Cells were treated with vehicle or WMJ‐8‐B at the indicated concentrations for 24 h. The percentage of PI‐stained cells in subG1, G0/G1, S and G2/M phases was then analysed by flow cytometric analysis as described in the Methods section. Each column represents the mean ± SEM of five independent experiments. Statistically significant differences were determined using one‐way ANOVA, with Tukey's post hoc test. *P < 0.05, compared with the control group.

Article Snippet: MDA‐MB‐231, MCF‐7, T47D, 4T1 and Hs68 cell lines were obtained from the Bioresource Collection and Research Centre (Hsinchu, Taiwan).

Techniques: MTT Assay, Control, Western Blot, Staining